Details of the available projects

Life/Coordination-Complex Molecular Science

Project Title: Operation and Design Principles of Motor Proteins Elucidated by Single-Molecule Techniques
Professor: Ryota IINO
http://groups.ims.ac.jp/organization/iino_g/index_e.html

Details of the project:

1) Generation of motor protein mutants, purification of motor proteins.
2) Single-molecule fluorescence imaging of linear motor proteins
3) Single-molecule imaging and manipulation of rotary motor proteins

References:

Anal. Chem. 2015, 87, 2079-2086; Biochemistry 2015, 54, 472–480; J. Biol. Chem. 2014, 289, 31212-31223; J. Biol. Chem. 2014, 289, 14056-14065; IUBMB Life 2014, 66, 624-630; J. Biol. Chem. 2013, 288, 32700-32707; Curr. Opin. Struct. Biol. 2013, 23, 229-234.

Required skills:

Experience of either molecular biological, biochemical or biophysical experiment of DNA or proteins is preferred.