Research Theme
Engineering of PET hydrolase for plastic recycling and environmental remediation, and engineering of plastic binding domains for detection and quantification of particles
Keywords
solid-liquid interfacial reaction, protein-engineering, single-molecule observation
Plastics are essential material for our modern life. To reduce the amount of petroleum and save the environment, we impruve the plastic degrading enzymes for plastic recycling and bioremediation by structural guided mutagenesis. The mutants were evaluated not only by biochemical analysis and X-ray crystallography, but also single molecule analysis to reveal improved kinetic parameters. Additionally, we engineer plastic specific binding domains to detect and quantify particles in environments. The easy detection system will be helpful to monitor the continuous trends of pollution and evaluate the antipollution measures.
Fig. 1 Scheme of PET hydrolase screening
Fig.2 An image structure of plastic detecting domain with fluorescence protein.
Selected Publications
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Akihiko Nakamura、Naoya Kobayashi、Nobuyasu Koga、Ryota Iino、「Positive Charge Introduction on the Surface of Thermostabilized PET Hydrolase Facilitates PET Binding and Degradation」ACS Catalysis, vol.11、2021年、P8550-8564
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Akihiko Nakamura、Daiki Ishiwata、Akasit Visootsat、Taku Uchiyama、Kenji Mizutani、Satoshi Kaneko、Takeshi Murata、Kiyohiko Igarashi、Ryota Iino、「Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases」 Journal of Biological Chemistry、vol.295、2020年、P14606～14617
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Akihiko Nakamura、Kei-ichi Okazaki、Tadaomi Furuta、Minoru Sakurai、Ryota Iino、「Processive chitinase is Brownian monorail operated by fast catalysis after peeling rail from crystalline chitin」Nature Communications、vol.9、2018年、P3814
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Akihiko Nakamura、Takuya Ishida、Katsuhiro Kusaka、Taro Yamada、Shinya Fushinobu、Ichiro Tanaka、Satoshi Kaneko、Kazunori Ohta、Hiroaki Tanaka、Koji Inaka、Yoshiki Higuchi、Nobuo Niimura、Masahiro Samejima、Kiyohiko Igarashi、「“Newton’s cradle” proton relay with amide-imidic acid tautomerization in inverting cellulase visualized by neutron crystallography」Science Advances vol.1、2015年、pe1500263
