Research Theme

Solid-State NMR for Molecular Science

Keywords

Solid State NMR, Biomolecules, Developments

In order to elucidate functions of molecules, characterizations of the molecules are essential. There are varieties of important molecules, which are insoluble to any solvents and functional at amorphous state. Solid-state NMR enables to obtain variety of information at atomic resolution without damages of molecules and significant restrictions. Thus, solid-state NMR is one of the essential tools for the characterizations of those molecules.

We have been working for methodology developments of solid-state NMR such as the analyses of error factors of precise distance measurements¹⁾, the developments of techniques for sensitivity enhancement without sample heating²⁾ and 2D correlation techniques to obtain orientational information of molecules³⁾. In addition, as studies for structural biology, we have been working for functional and structural characterizations of membrane proteins and peptides such as ⁺H channel from influenza virus A⁴⁾, a peripheral membrane protein of phospholipase C-δ1 PH domain⁵⁾, and amyloid-β oligomers induced on lipid membranes⁶⁾. Furthermore, as studies for material science, we have been working for characterizations of variety of molecular materials such as silk⁷⁾, and supramolecules based on synthetic polymers⁸⁾ through collaborations with several research groups.

Outline of our studies.

Selected Publications

1. K. Nishimura*, A. Naito.“REDOR in Multiple Spin System”, Modern Magnetic Resonance, Springer, The Netherlands (2006).
2. K. Nishimura*, A. Naito, Chem. Phys. Lett. 380, 569-576 (2003).
3. K. Nishimura*, A. Naito, Chem. Phys. Lett. 402, 245-250 (2005).
4. K. Nishimura, S. Kim, L. Zhang, T. A. Cross, Biochemistry. 41, 13170-13177 (2002).
5. N. Uekama, T. Aoki, T. Maruoka, S. Kurisu, A. Hatakeyama, S. Yamaguchi, M. Okada, H. Yagisawa, K. Nishimura*, S. Tuzi*, Biochim. Biophys. Acta 1788, 2575-2583 (2009).
6. M. Yagi-Utsumi, K. Kato, and K. Nishimura*, PlosONE 11, 0146405 (1-10) (2016).
7. T. Asakura, T. Ohota, S. Kametani, K. Okushita, K. Yazawa, Y. Nishiyama, K. Nishimura, A. Aoki, F. Suzuki, H. Kaji, A. Ulrich, M. Williamson, Macromolecules 48, 28-36 (2015).
8. N. Ousaka, F. Mamiya, Y. Iwata, K. Nishimura, and E. Yashima, Angew. Chem. Int. Ed. 56, 791-795 (2017)