研究・研究者
研究会・セミナー
| 演 題 | 「Visualizing Protein Structural Dynamics with Picosecond X-ray Crystallography and Liquidography (Solution Scattering)」 |
|---|---|
| 日 時 | 2011年09月27日(火) 16:00 |
| 講演者 | Prof. Hyotcherl Ihee（Department of Chemistry, KAIST） |
| 場 所 | 分子科学研究所 研究棟201号室 |
| 概 要 |
The principle, experimental technique, data analysis, and applications of time-resolved X-ray diffraction and scattering to study spatiotemporal reaction dynamics of proteins in single crystals and solutions will be presented. X-ray crystallography, the major structural tool to determine 3D structures of proteins, can be extended to time-resolved X-ray crystallography with a laser-excitation and X-ray-probe scheme, and all the atomic positions in a protein can be tracked during their biological function. However time-resolved Crystallography has been limited to a few model systems with reversible photocycles due to the stringent prerequisites such as highly-ordered and radiation-resistant single crystals and crystal packing constraints might hinder biologically relevant motions. These problems can be overcome by applying time-resolved X-ray diffraction directly to protein solutions rather than protein single crystals. To emphasize that structural information can be obtained from the liquid phase, this time-resolved X-ray solution scattering technique is named time-resolved X-ray liquidography (TRXL) in analogy to time-resolved X-ray crystallography where the structural information of reaction intermediates is obtained from the crystalline phase. We demonstrate tracking of protein’s structural changes in solution using TRXL. |
| お問合せ先 | 斉藤真司（理論・計算分子科学研究領域） |
