研究・研究者
研究会・セミナー
| 日 時 | 2012年10月18日(木) 16:00 |
|---|---|
| 講演者 | Prof. Stephane Menage (Univ. of Grenoble) |
| 場 所 | 山手２号館３階 共通セミナー室 |
| 概 要 | The presentation deals with the design of original metalloenzymes for dioxygen activation using our own technology based based on the insertion of inorganic catalysts into the periplasmic nickel-binding protein NikA via supramolecular interactions to convert it from a transport protein to a metalloenzyme. First, we will discuss a new concept for the understanding of catalysis mechanism using the combination of protein X-ray crystallography and inorganic catalysis. The targeted reaction concerns the aromatic hydroxylation by dioxygen catalyzed by an iron complex. Second, a new type of oxidase will be presented, using iron complexes known as catalysts for C-H, C=C, C-OH and oxygen transfer to sulfides. Finally, we have developed a new method to create new enzyme based on the search for sulfides enzyme substrates using an original screening approach. This work highlighted the importance of the synergetic effect of the different partners, i.e. the protein, the inorganic complex and the substrate. |
| お問合せ先 | 青野重利（aono@ims.ac.jp、内線 5575) |
