研究・研究者
研究会・セミナー
| 演 題 | 「Novel IR spectroscopies to study biological membranes and membrane proteins」 |
|---|---|
| 日 時 | 2014年10月14日(火) 10:30 より 12:00 まで |
| 講演者 | Joachim Heberle 教授 (Free University of Berlin) |
| 場 所 | 分子科学研究所 研究棟201セミナー室 |
| 概 要 |
Membrane proteins are the target of more than 50% of all drugs and are encoded by about 30% of the human genome. Electrophysiological techniques, like patch-clamp, unravelled many functional aspects of membrane proteins but suffer from structural sensitivity. We have developed Surface Enhanced Infrared Difference Absorption Spectroscopy (SEIDAS) to probe potential-induced structural changes of a protein on the level of a monolayer (see 1 for a recent review). A novel concept is introduced to incorporate membrane proteins into solid supported lipid bilayers in an orientated manner via the affinity of the His-tag to the Ni-NTA terminated gold surface. General applicability of the methodological approach is shown by tethering photosystem II to the gold surface. In conjunction with hydrogenase, the basis is set towards a biomimetic system for H2-production. FTIR difference spectra of a monolayer of sensory rhodopsin II were recorded under voltage-clamp conditions. This approach opens an avenue towards mechanistic studies of voltage-gated ion channels with unprecedented structural and temporal sensitivity. Finally, scanning near-field IR micrososcopy will be introduced and applied to study the structure of biomembranes2. References:
1. Ataka, K., Stripp, S., and Heberle, J. Biochim. Biophys. Acta 1828, 2283-93 (2013) |
| その他 |
