研究・研究者
研究会・セミナー
| 演 題 | 「Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array」 |
|---|---|
| 日 時 | 2019年08月23日(金) 16:00 より 17:00 まで |
| 講演者 | 張 翼 博士 （国立研究開発法人海洋研究開発機構 超先鋭研究開発部門 研究員） |
| 場 所 | 山手3号館2階共通セミナー室 |
| 概 要 |
This presentation reports a general strategy based on Poisson statistics that enables a rapid and efficient acquisition of enzyme mutants from just a few clones within a day. We prepared a high-density femtoliter droplet array (FemDA) consisting of one million uniform droplets per 1 cm2 to carry out high-throughput protein synthesis and screening. Single DNA molecules from a mutagenesis library were randomly distributed into each droplet following a Poisson process to initiate the protein synthesis, and then can be recovered by a microcapillary. The protein yield in each droplet was proportional to the number of DNA molecules, meaning that droplets with apparent intensities higher than the Poisson distribution-predicted maximum can be readily identified as the exact hits exhibiting the desired increased activity, rather than a co-encapsulation of multiple DNA molecules. This unique strategy combining the miniaturized device technology and the mathematical modeling enabled cost-effective screening of several kinds of novel alkaline phosphatases with remarkably improved activities from just a few droplets using less than 10 nL reagents. |
| その他 |
協賛：新学術領域研究「発動分子科学」 |
| お問合せ先 | 飯野亮太（内線5230） |
